Identification and virulence of fungi associated with invasive plant species of water hyacinth in Guilan wetland ecosystems

Document Type : Article

Authors

1 Academic member of Guilan research and education center of Agriculture and natural resources-Rasht.

2 fishes

Abstract

During 2017-18, in order to identifying and comparing of virulence of fungi associated with common water hyacinth (Eichhornia crassipes L.), an aquatic and invasive plant, 58 plant samples (leaf, petiole and stem) were collected from wetland ecosystems of Guilan province (northern Iran) and studied in laboratory. A total of 31 fungal isolates with different frequencies including Alternaria alternata (48.39%), Curvularia lunata (16.13%), Colletotrichum lindemutianum (6.45%), Phoma sp. (6.45%), Bipolaris victoriae (9.68%) and Fusarium semitectum (12.9%) were isolated and identified. Pathogenic responses and/or virulence of fungi were rated according to the width of the leaf lesion [(++ >5mm highly pathogenic, +3-5mm pathogenic, -10 d: low virulence]. The results showed that A. alternata and C. lunata were highly virulent to water hyacinth under laboratory and greenhouse conditions. However, the wide host range and non-specific phytopathogenic effects of A. alternata and C. lunata on the economically important plants such as rice (Oryza sativa L.) precluded their use.

Keywords


1-      Ainsworth, G.C., Sparrow, F.K. and Sussman, A.S., 1973. The Fungi, Vol. IVA. A: A taxonomic review with keys: ascomycetes and fungi imperfecti. Academic Press, New York, NY.
2-      Aneja, K.R. and Singh, K., 1989. Alternaria alternata (Fr.) Keissler, a pathogen of waterhyacinth with biocontrol potential. International Journal of Pest Management35(4), pp.354-356.
3-      Aneja, K.R., 2003. Experiments in microbiology, plant pathology and biotechnology. New Age International.. 4th ed. New Delhi: New Age International (p) Ltd. Publishers.
4-      Aneja, K.R., Kumar, P. and Sharma, C., 2014. A new strain of Alternaria alternata (AL-14) on water hyacinth from India. Journal of Innovative Biology June1(2), pp.117-121.
5-      Auld, B.A. and Morin, L., 1995. Constraints in the development of bioherbicides. Weed Technology9(3), pp.638-652.
6-      Babu, R.M., Sajeena, A., Seetharaman, K., Vidhyasekaran, P., Rangasamy, P., Raja, A.S. and Biji, K.R., 2002. Host range of Alternaria alternata—a potential fungal biocontrol agents for waterhyacinth in India. Crop protection21(10), pp.1083-1085
7-      Babu, R.M., Sajeena, A. and Seetharaman, K., 2003. Bioassay of the potentiality of Alternaria alternata (Fr.) Keissler as a bioherbicide to control waterhyacinth and other aquatic weeds. Crop Protection22(8), pp.1005-1013.
8-      Bateman, R., 2000, October. IMPECCA: an international, collaborative program to investigate the development of a mycoherbicide for use against water hyacinth in Africa. In ACIAR PROCEEDINGS (pp. 57-61). ACIAR; 1998.
9-      Booth, C., 1977. Fusarium. Laboratory guide to the identification of the major species. Commonwealth Mycological Institute. Kew, Surrey, England.
10-   Burgess, L.W., Summerell, B.A., Bullock, S., Gott, K.P. and Backhouse, D., 1994. Laboratory Manual for Fusarium Research. Fusarium Research Laboratory, Department of Crop Sciences, University of Sydney, and The Royal Botanic Gardens, Sydney. New South Wales.
11-    Charudattan, R. and Conway, K.E., 1975. Comparison of Uredo eichhorniae, the waterhyacinth rust, and Uromyces pontederiae. Mycologia67(3), pp.653-657.

12-   Charudattan, R., Linda, S.B., Kluepfel, M. and Osman, Y.A., 1985. Biocontrol efficacy of Cercospora rodmanii on waterhyacinth. Phytopathology, 75(11), pp.1263-1269.

13-   Charudattan, R., 1986. Cercospora rodmannii: a biological control agent for water hyacinth. Aquatics, 8, pp.21- 24.

14-   Charudattan, R., 1986. Integrated control of waterhyacinth (Eichhornia crassipes) with a pathogen, insects, and herbicides. Weed Science, 34(S1), pp.26-30.

15-   Charudattan, R., 2001. Biological control of weeds by means of plant pathogens: significance for integrated weed management in modern agro-ecology. BioControl46(2), pp.229-260.
16-  Charudattan, R. 2005. Ecological, practical and political inputs into selection of weed targets: what makes a good biological control target? Biological Control, 35, pp.183-196.
17-  Cruttwell, M.R.E., 2000. Successes in biological control of weeds. In: Spencer N.R., ed. Proceedings of the 10th International Symposium on Biological Control of Weeds, 4-14 July 1999, Montana State University, Bozeman, Montana, USA, 3-14.
18-  Cuda, J. P., Charudattan, R., Grodowitz, M. J., Newman, R. M., Shearer, J. F., Tamayo, M. L. and Villegas B. 2008. Recent advances in biological control of submersed aquatic weeds. Journal of Aquatic Plant Management, 46, pp.15–32.
19-   Dagno, K., 2006. Evaluation des microorganismes fongiques en tant qu’agents de lutte biologique contre Eichhornia crassipes (Martius) Solms-Laubach dans le bassin du fleuve Niger au Mali. DEA Doc. Sci. agron. Gembloux, Belgique: Gembloux Agricultural University–FUSAGx.
20-  Dagno, K., Lahlali, R., Diourté, M. and Jijakli, M.H., 2011. Effect of temperature and water activity on spore germination and mycelial growth of three fungal biocontrol agents against water hyacinth (Eichhornia crassipes). Journal of applied microbiology110(2), pp.521-528.
21-  Dagno, K., Lahlali, R., Diourté, M. and Jijakli, H., 2011. Production and oil-emulsion formulation of Cadophora malorum and Alternaria jacinthicola, two biocontrol agents against water hyacinth (Eichhornia crassipes). African Journal of Microbiology Research5(8), pp.924-929.
22-  Dagno, K., Lahlali, R., Diourté, M. and Jijakli, H., 2012. Present status of the development of mycoherbicides against water hyacinth: successes and challenges. A review. Biotechnologie, Agronomie, Société et Environnement16(3), pp.360-368.
23-  de Jong, M.D. and de Voogd, W.B., 2006. Novel mycoherbicides for biological control of aquatic weeds such as Water Hyacinth and Water Lettuce©: Patent Disclosure. Annals of Plant Protection Sciences14(1), pp.274-274.
24-  El-Morsy, E.M., Dohlob, S.M. and Hyde, K.D., 2006. Diversity of Alternaria alternata a common destructive pathogen of Eichhornia crassipes in Egypt and its potential use in biological control. Fungal Divers23, pp.139-158.
25-  El-Morsy, E.M., 2004. Evaluation of microfungi for the biological control of water hyacinth in Egypt. Fungal Divers16, pp.35-51.
26-  Evans, H.C. and Reeder, R.H., 2000, October. Fungi associated with Eichhornia crassipes (water hyacinth) in the upper Amazon basin and prospects for their use in biological control. In ACIAR PROCEEDINGS (pp. 62-70). ACIAR; 1998.
27-  Freeman, T.E. and Charudattan, R., 1984. Cercospora rodmanii Conway--a biocontrol agent for waterhyacinth. Bulletin-Florida, Agricultural Experiment Stations.
28-  Hasan, S. and Ayres, P.G., 1990. The control of weeds through fungi; principles and prospects. New Phytologist115(2), pp.201-222.
29-  Holliday P., 1993. A dictionary of plant pathogens. Cambridge University Press. New Delhi. 369 p.
30-  Lacap, D.C., Hyde, K.D. and Liew, E.C.Y., 2003. An evaluation of the fungal'morphotype'concept based on ribosomal DNA sequences. Fungal Diversity.
31-  Leslie, J.F. and Summerell, B.A. 2006. The Fusarium Laboratory Manual. Blackwell, Oxford, 388 p. 
32-  Jimenez, M.M. and Balandra, M.A.G., 2007. Integrated control of Eichhornia crassipes by using insects and plant pathogens in Mexico. Crop Protection26(8), pp.1234-1238.
33-  Naseema, A., Praveena, R., Nair, R.R. and Peethambaran, C.K., 2004. Fusarium pallidoroseum for management of water hyacinth. Current Science86(6), pp.770-771.
34-  Mutebi, C.M., Arama, P.F., Opande, G.O., Were, J.O., Omolo, P.O. and Buyela, D., 2013. Innundative biocontrol of water hyacinth (Eichhornia crassipes (Martias Solms) Laubach) using zonate leafspot (Acremonium zonatum Sawada Gams) fungal agent. 11th African Crop Science Proceedings, Sowing innovations for sustainable food and nutrition security in Africa. Entebbe, Uganda, 14-17 October, 2013, pp.413-415.
35-  Praveena, R. and Naseema, A., 2006. Fungi occurring on water hyacinth [Eichhornia crassipes (Mart.) Solms] in Kerala. Journal of Tropical Agriculture42, pp.21-23.
36-  Rajesha, G., Mantur, S.G., Shankar, M.R., Boranayaka, M.B. and Shadakshari, T.V., 2010. In vitro evaluation of fungicides and biocontrol agents against Colletotrichum lindemuthianum causing anthracnose of Dolichos bean. International Journal of Plant Protection3(1), pp.114-116.
37-  Puja, R. and Pandey, A.K., 2008. Efficacy of pathogens of water hyacinth (Eichhornia crassipes) singly and in combination for its biological control. Journal of Biological Control22(1), pp.173-177.
38-  Menon, G.I. and Sinha, S., 2007. Biological control of insect pests and diseases of forestry importance. Current Science92(2), p.166.
39-  Shabana, Y.M., 2005. The use of oil emulsions for improving the efficacy of Alternaria eichhorniae as a mycoherbicide for waterhyacinth (Eichhornia crassipes). Biological Control32(1), pp.78-89.
40-  Shabana, Y.M., 1997. Formulation of Alternaria eichhorniae, a mycoherbicide for waterhyacinth, in invert emulsions averts dew dependence/Formulierung von Alternaria eichhorniae, einem Mykoherbizid gegen die Wasserhyazinthe, in Wasser-in-Öl-Emulsionen verhindert die Abhāngigkeit von Tau. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz/Journal of Plant Diseases and Protection, pp.231-238.
41-  Shabana, Y.M., Charudattan, R. and Elwakil, M.A., 1995. First record of Alternaria eichhorniae and Alternaria alternata on waterhyacinth(Eichhornia crassipes) in Egypt. Plant Disease79(3), p.319.
42-  Shabana, Y.M., Charudattan, R. and Elwakil, M.A., 1995. Identification, pathogenicity, and safety of Alternaria eichhorniae from Egypt as a bioherbicide agent for waterhyacinth. Biological Control5(2), pp.123-135.
43-  Shabana, Y.M., Charudattan, R. and Elwakil, M.A., 1995. Evaluation of Alternaria eichhorniae as a bioherbicide for waterhyacinth (Eichhornia crassipes) in greenhouse trials. Biological Control5(2), pp.136-144.
44-  Shearer, J.F., 2010. A historical perspective of pathogen biological control of aquatic plants. Weed Technology24(2), pp.202-207.
45-  Simmons, E.G., 2007. Alternaria: An Indentification Manual(No. PA 632.488 S56.).
46-  Sivanesan, A., 1987. Graminicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and their teleomorphs. CAB International.
47-  Sutton, B.C., 1980. The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. Commonwealth Mycological Institute..
48-  TeBeest, D.O., 1991. Microbial control of weeds. New York, USA: Chapman and Hall.